PrimePCR™ Probe Assay: Gabarapl2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0023129
Assay Design:   exonic
Chromosome Location:   19:54982191-54982297question
Amplicon Length:   77
Splice Variants Targeted:   ENSRNOT00000048998

Gene Information

mouse homolog may function as a soluble transport factor and/or a GABA(A) receptor linker [RGD, Feb 2006]

Gene Symbol:   Gabarapl2
Gene Name:   Gamma-aminobutyric acid receptor-associated protein-like 2
Aliases:   Not Available
RefSeq:   NM_022706
Ensembl:   ENSRNOG00000019425
Entrez:   64670
UniGene:   Rn.64537
Chromosome Mapping:   19q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.920000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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