PrimePCR™ Probe Assay: Cyp1b1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0022934
Assay Design:   exonic
Chromosome Location:   6:2300289-2300400question
Amplicon Length:   82
Splice Variants Targeted:   ENSRNOT00000066363

Gene Information

plays a role in polycyclic aromatic hydrocarbon metabolism in adrenal microsomes [RGD, Feb 2006]

Gene Symbol:   Cyp1b1
Gene Name:   Cytochrome P450 1B1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000040287
Entrez:   25426
UniGene:   Rn.10125
Chromosome Mapping:   6q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.430000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download