PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000050912, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0056965
Assay Design:   Exonic
Chromosome Location:   6:147625808-147625922question
Amplicon Length:   85
Splice Variants Targeted:   ENSRNOT00000040773 ENSRNOT00000009295 ENSRNOT00000064924 ENSRNOT00000042118 ENSRNOT00000073304 ENSRNOT00000041706 ENSRNOT00000050297 ENSRNOT00000042728 ENSRNOT00000052021 ENSRNOT00000075175 ENSRNOT00000075034 ENSRNOT00000075363 ENSRNOT00000070939 ENSRNOT00000071714 ENSRNOT00000073820 ENSRNOT00000074400 ENSRNOT00000072633 ENSRNOT00000072900 ENSRNOT00000071287 ENSRNOT00000072238 ENSRNOT00000048463 ENSRNOT00000071667 ENSRNOT00000066416 ENSRNOT00000017240 ENSRNOT00000015421

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000050912
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050912
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 38.600000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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