PrimePCR™ SYBR® Green Assay: DCM5, Rat

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0056674
Assay Design:   Exonic
Chromosome Location:   2:253619920-253620051question
Amplicon Length:   102
Splice Variants Targeted:   ENSRNOT00000020697 ENSRNOT00000050902 ENSRNOT00000071992 ENSRNOT00000061249 ENSRNOT00000026279 ENSRNOT00000073834 ENSRNOT00000072316 ENSRNOT00000028997 ENSRNOT00000010753 ENSRNOT00000057989 ENSRNOT00000047380 ENSRNOT00000030065 ENSRNOT00000059522 ENSRNOT00000008747 ENSRNOT00000038939 ENSRNOT00000005089 ENSRNOT00000072612 ENSRNOT00000073892 ENSRNOT00000062027 ENSRNOT00000026405 ENSRNOT00000002036 ENSRNOT00000073197 ENSRNOT00000072216

Gene Information

Description Not Available

Gene Symbol:   DCM5
Gene Name:   DCM5 protein; RCG28429; Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048400
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.994800
y-intercept 38.870000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.