PrimePCR™ SYBR® Green Assay: RGD1566270, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0056622
Assay Design:   Exonic
Chromosome Location:   3:64497871-64498001question
Amplicon Length:   101
Splice Variants Targeted:   ENSRNOT00000001265 ENSRNOT00000012902 ENSRNOT00000050067 ENSRNOT00000056321 ENSRNOT00000046791 ENSRNOT00000071210 ENSRNOT00000049138 ENSRNOT00000048673 ENSRNOT00000058595 ENSRNOT00000048420 ENSRNOT00000050803 ENSRNOT00000071995 ENSRNOT00000041479 ENSRNOT00000050291 ENSRNOT00000042619 ENSRNOT00000051772 ENSRNOT00000049571 ENSRNOT00000048356 ENSRNOT00000049363 ENSRNOT00000050384 ENSRNOT00000048123 ENSRNOT00000048115 ENSRNOT00000054860 ENSRNOT00000051901 ENSRNOT00000040249 ENSRNOT00000040493 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RGD1566270
Gene Name:   Protein RGD1566270
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048003
Entrez:   296502
UniGene:   Not Available
Chromosome Mapping:   3q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.995800
y-intercept 36.700000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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