PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000038057, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0056243
Assay Design:   Exonic
Chromosome Location:   19:69111599-69111815question
Amplicon Length:   187
Splice Variants Targeted:   ENSRNOT00000011793 ENSRNOT00000034681 ENSRNOT00000030268 ENSRNOT00000037100 ENSRNOT00000011801 ENSRNOT00000032631 ENSRNOT00000044716 ENSRNOT00000011239 ENSRNOT00000037571 ENSRNOT00000037416 ENSRNOT00000014849 ENSRNOT00000065387 ENSRNOT00000050117 ENSRNOT00000057412 ENSRNOT00000042570 ENSRNOT00000047001 ENSRNOT00000048860 ENSRNOT00000043027 ENSRNOT00000049689

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000038057
Gene Name:   Protein LOC100362277
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000038057
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 37.040000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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