PrimePCR™ SYBR® Green Assay: Crtap, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0054926
Assay Design:   Exonic
Chromosome Location:   8:121734185-121734290question
Amplicon Length:   76
Splice Variants Targeted:   ENSRNOT00000075435 ENSRNOT00000065013 ENSRNOT00000025354 ENSRNOT00000041665 ENSRNOT00000011832 ENSRNOT00000041777 ENSRNOT00000040029 ENSRNOT00000037439 ENSRNOT00000048245 ENSRNOT00000041577 ENSRNOT00000005023 ENSRNOT00000012334 ENSRNOT00000031056 ENSRNOT00000022839 ENSRNOT00000026941 ENSRNOT00000014158 ENSRNOT00000006789 ENSRNOT00000042983 ENSRNOT00000022233 ENSRNOT00000017618 ENSRNOT00000075524 ENSRNOT00000005775 ENSRNOT00000038088 ENSRNOT00000054987 ENSRNOT00000059814 ENSRNOT00000035385 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Crtap
Gene Name:   Cartilage-associated protein precursor
Aliases:   Not Available
RefSeq:   NM_001108785
Ensembl:   ENSRNOG00000048343
Entrez:   363158
UniGene:   Rn.229070
Chromosome Mapping:   8q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.290000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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