PrimePCR™ SYBR® Green Assay: Olr1265, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0054825
Assay Design:   Exonic
Chromosome Location:   2:65254791-65254925question
Amplicon Length:   105
Splice Variants Targeted:   ENSRNOT00000073106 ENSRNOT00000075754 ENSRNOT00000075218 ENSRNOT00000045736 ENSRNOT00000073532 ENSRNOT00000073166 ENSRNOT00000071850 ENSRNOT00000074552 ENSRNOT00000045171 ENSRNOT00000000986 ENSRNOT00000074056 ENSRNOT00000074742 ENSRNOT00000073159 ENSRNOT00000075359 ENSRNOT00000073523 ENSRNOT00000050970 ENSRNOT00000072310 ENSRNOT00000075604 ENSRNOT00000051165 ENSRNOT00000047107 ENSRNOT00000048537 ENSRNOT00000074265 ENSRNOT00000075675

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr1265
Gene Name:   Olfactory receptor Olr1265
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000047572
Entrez:   300584
UniGene:   Rn.143508
Chromosome Mapping:   8q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 38.280000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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