PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000046767, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0054737
Assay Design:   Exonic
Chromosome Location:   10:110999788-110999893question
Amplicon Length:   76
Splice Variants Targeted:   ENSRNOT00000071844 ENSRNOT00000073174 ENSRNOT00000040119 ENSRNOT00000073222 ENSRNOT00000074792 ENSRNOT00000074699 ENSRNOT00000042693 ENSRNOT00000073754 ENSRNOT00000062058 ENSRNOT00000050916 ENSRNOT00000034630 ENSRNOT00000074428 ENSRNOT00000073410 ENSRNOT00000044505 ENSRNOT00000075323 ENSRNOT00000072621 ENSRNOT00000074130 ENSRNOT00000072076 ENSRNOT00000074598 ENSRNOT00000048946 ENSRNOT00000074402 ENSRNOT00000071037 ENSRNOT00000043877 ENSRNOT00000070942 ENSRNOT00000073032 ENSRNOT00000072232 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000046767
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046767
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 37.290000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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