PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000046206, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0054671
Assay Design:   Exonic
Chromosome Location:   2:20730045-20730202question
Amplicon Length:   128
Splice Variants Targeted:   ENSRNOT00000070996 ENSRNOT00000073826 ENSRNOT00000074316 ENSRNOT00000073881 ENSRNOT00000075488 ENSRNOT00000073600 ENSRNOT00000071279 ENSRNOT00000072358 ENSRNOT00000071520 ENSRNOT00000070839 ENSRNOT00000075660 ENSRNOT00000071813 ENSRNOT00000075737 ENSRNOT00000072499 ENSRNOT00000072650 ENSRNOT00000071379 ENSRNOT00000071967 ENSRNOT00000048690 ENSRNOT00000073223 ENSRNOT00000070982 ENSRNOT00000072846 ENSRNOT00000072987 ENSRNOT00000071864 ENSRNOT00000072515 ENSRNOT00000072148 ENSRNOT00000075354 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000046206
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046206
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 37.560000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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