PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000045862, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0054627
Assay Design:   Exonic
Chromosome Location:   1:265927944-265928109question
Amplicon Length:   117
Splice Variants Targeted:   ENSRNOT00000072228 ENSRNOT00000070996 ENSRNOT00000072012 ENSRNOT00000072688 ENSRNOT00000074151 ENSRNOT00000073826 ENSRNOT00000071695 ENSRNOT00000074787 ENSRNOT00000074316 ENSRNOT00000048832 ENSRNOT00000049456 ENSRNOT00000073881 ENSRNOT00000071140 ENSRNOT00000071352 ENSRNOT00000075615 ENSRNOT00000075533 ENSRNOT00000072361 ENSRNOT00000072079 ENSRNOT00000075488 ENSRNOT00000073190 ENSRNOT00000072157 ENSRNOT00000074064 ENSRNOT00000073600 ENSRNOT00000074940 ENSRNOT00000072415 ENSRNOT00000072758 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000045862
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000045862
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.520000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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