PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000023436, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0054196
Assay Design:   Exonic
Chromosome Location:   8:73224074-73224267question
Amplicon Length:   164
Splice Variants Targeted:   ENSRNOT00000011793 ENSRNOT00000034681 ENSRNOT00000000060 ENSRNOT00000035658 ENSRNOT00000032995 ENSRNOT00000044716 ENSRNOT00000011239 ENSRNOT00000044706 ENSRNOT00000016784 ENSRNOT00000049793 ENSRNOT00000011002 ENSRNOT00000029388 ENSRNOT00000014167 ENSRNOT00000051929 ENSRNOT00000019545 ENSRNOT00000002883 ENSRNOT00000024402 ENSRNOT00000002917 ENSRNOT00000048888 ENSRNOT00000033374

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000023436
Gene Name:   Protein RGD1565455
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000023436
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 37.760000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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