PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000017139, Rat

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0054092
Assay Design:   Exonic
Chromosome Location:   2:208919220-208919335question
Amplicon Length:   75
Splice Variants Targeted:   ENSRNOT00000064632 ENSRNOT00000023364 ENSRNOT00000036706 ENSRNOT00000032406 ENSRNOT00000045607 ENSRNOT00000043945 ENSRNOT00000039569 ENSRNOT00000035566 ENSRNOT00000032908 ENSRNOT00000003755 ENSRNOT00000035216 ENSRNOT00000035594 ENSRNOT00000000455 ENSRNOT00000040427 ENSRNOT00000048151 ENSRNOT00000044035 ENSRNOT00000037429 ENSRNOT00000040732 ENSRNOT00000064141 ENSRNOT00000036106 ENSRNOT00000049603 ENSRNOT00000037885 ENSRNOT00000041930 ENSRNOT00000023343 ENSRNOT00000026712 ENSRNOT00000051417 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000017139
Gene Name:   Glyceraldehyde-3-phosphate dehydrogenase
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000017139
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 38.490000
Efficiency 85

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.