PrimePCR™ SYBR® Green Assay: Tmem191c, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0053813
Assay Design:   Exonic
Chromosome Location:   11:91030661-91030932question
Amplicon Length:   60
Splice Variants Targeted:   ENSRNOT00000002866 ENSRNOT00000015179 ENSRNOT00000075767 ENSRNOT00000064947 ENSRNOT00000017854 ENSRNOT00000002546 ENSRNOT00000048474 ENSRNOT00000022742 ENSRNOT00000016557 ENSRNOT00000072263 ENSRNOT00000068102 ENSRNOT00000042301 ENSRNOT00000000084 ENSRNOT00000047601 ENSRNOT00000046956 ENSRNOT00000022383 ENSRNOT00000026749 ENSRNOT00000021114

Gene Information

Description Not Available

Gene Symbol:   Tmem191c
Gene Name:   Protein LOC100364951
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000001864
Entrez:   680867
UniGene:   Not Available
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 38.050000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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