PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000030586, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0053645
Assay Design:   Exonic
Chromosome Location:   8:90795709-90795858question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000021596 ENSRNOT00000015104 ENSRNOT00000020032 ENSRNOT00000066710 ENSRNOT00000026518 ENSRNOT00000022836 ENSRNOT00000021725 ENSRNOT00000036959 ENSRNOT00000011041 ENSRNOT00000001164 ENSRNOT00000041852 ENSRNOT00000005876 ENSRNOT00000056170 ENSRNOT00000008387 ENSRNOT00000041388 ENSRNOT00000032861 ENSRNOT00000034305 ENSRNOT00000067142 ENSRNOT00000043474 ENSRNOT00000022426 ENSRNOT00000027470 ENSRNOT00000041210 ENSRNOT00000003002 ENSRNOT00000042736

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000030586
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000030586
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.996100
y-intercept 37.960000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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