PrimePCR™ SYBR® Green Assay: Lrrc7, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Lrrc7, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Lrrc7, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0053193
Assay Design:   Exonic
Chromosome Location:   2:283610243-283610375question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000028149 ENSRNOT00000016453 ENSRNOT00000066024 ENSRNOT00000052190 ENSRNOT00000016073 ENSRNOT00000033502

Gene Information

targets the calcium/calmodulin-dependent protein kinase II to the postsynaptic density and may participate in specific adhesion between presynaptic and postsynaptic membranes at glutamatergic synapses [RGD, Feb 2006]

Gene Symbol:   Lrrc7
Gene Name:   Leucine-rich repeat-containing protein 7
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000011980
Entrez:   117284
Chromosome Mapping:   2q45

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.580000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download