PrimePCR™ SYBR® Green Assay: Olr1280, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0053050
Assay Design:   Exonic
Chromosome Location:   2:134771365-134771479question
Amplicon Length:   85
Splice Variants Targeted:   ENSRNOT00000073239 ENSRNOT00000026287 ENSRNOT00000039191 ENSRNOT00000073289 ENSRNOT00000051439 ENSRNOT00000017141 ENSRNOT00000030891 ENSRNOT00000022089 ENSRNOT00000023153 ENSRNOT00000064518 ENSRNOT00000027385 ENSRNOT00000071442 ENSRNOT00000028389 ENSRNOT00000026149 ENSRNOT00000055251 ENSRNOT00000005839 ENSRNOT00000020994 ENSRNOT00000073657 ENSRNOT00000031717 ENSRNOT00000075355

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr1280
Gene Name:   Olfactory receptor Olr1280
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050953
Entrez:   315571
UniGene:   Rn.85601
Chromosome Mapping:   2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 37.000000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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