PrimePCR™ SYBR® Green Assay: Avpr1b, Rat

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0051989
Assay Design:   Exonic
Chromosome Location:   13:53443985-53444084question
Amplicon Length:   70
Splice Variants Targeted:   ENSRNOT00000025377 ENSRNOT00000074512 ENSRNOT00000074204 ENSRNOT00000066017 ENSRNOT00000048564 ENSRNOT00000057386 ENSRNOT00000031231 ENSRNOT00000074380 ENSRNOT00000045573 ENSRNOT00000051404 ENSRNOT00000028348 ENSRNOT00000023920

Gene Information

may play a role in the regulation of cerebrospinal fluid water content [RGD, Feb 2006]

Gene Symbol:   Avpr1b
Gene Name:   Vasopressin V1b receptor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048522
Entrez:   29462
UniGene:   Rn.10096
Chromosome Mapping:   13q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 38.170000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.