PrimePCR™ SYBR® Green Assay: MGC116197, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0051856
Assay Design:   Exonic
Chromosome Location:   9:8144557-8145349question
Amplicon Length:   95
Splice Variants Targeted:   ENSRNOT00000043153 ENSRNOT00000051066 ENSRNOT00000073775 ENSRNOT00000071982 ENSRNOT00000043269 ENSRNOT00000046852 ENSRNOT00000073673 ENSRNOT00000074599 ENSRNOT00000071620 ENSRNOT00000061476 ENSRNOT00000051488 ENSRNOT00000073169 ENSRNOT00000041304 ENSRNOT00000074550 ENSRNOT00000048862 ENSRNOT00000071886 ENSRNOT00000038077 ENSRNOT00000072533

Gene Information

Description Not Available

Gene Symbol:   MGC116197
Gene Name:   Uncharacterized protein LOC367620
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000033341
Entrez:   367620
UniGene:   Rn.155021
Chromosome Mapping:   9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.995900
y-intercept 37.420000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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