PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000029310, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0022418
Assay Design:   exonic
Chromosome Location:   5:148698607-148698795question
Amplicon Length:   159
Splice Variants Targeted:   ENSRNOT00000036333 ENSRNOT00000048646 ENSRNOT00000037123 ENSRNOT00000009844 ENSRNOT00000009816 ENSRNOT00000049151 ENSRNOT00000049436 ENSRNOT00000006754 ENSRNOT00000044551 ENSRNOT00000014590 ENSRNOT00000016409 ENSRNOT00000045509 ENSRNOT00000064857 ENSRNOT00000019331 ENSRNOT00000052141 ENSRNOT00000046365 ENSRNOT00000047694 ENSRNOT00000041927 ENSRNOT00000051043 ENSRNOT00000047511 ENSRNOT00000047713 ENSRNOT00000051387 ENSRNOT00000051207 ENSRNOT00000042764 ENSRNOT00000041410 ENSRNOT00000043491 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000029310
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000029310
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.380000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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