PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000047454, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0022032
Assay Design:   exonic
Chromosome Location:   3:173621393-173621607question
Amplicon Length:   67
Splice Variants Targeted:   ENSRNOT00000030857 ENSRNOT00000033772 ENSRNOT00000047433 ENSRNOT00000043099 ENSRNOT00000070851 ENSRNOT00000073416 ENSRNOT00000008101 ENSRNOT00000008047 ENSRNOT00000075257 ENSRNOT00000075429 ENSRNOT00000042110 ENSRNOT00000063989 ENSRNOT00000075012 ENSRNOT00000075542 ENSRNOT00000072202 ENSRNOT00000072043 ENSRNOT00000049457 ENSRNOT00000051082 ENSRNOT00000074655 ENSRNOT00000072350 ENSRNOT00000073650 ENSRNOT00000066393 ENSRNOT00000075091 ENSRNOT00000049165

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000047454
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000047454
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 36.690000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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