PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000030564, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000030564, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000030564, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0021781
Assay Design:   exonic
Chromosome Location:   1:42120049-42120161question
Amplicon Length:   83
Splice Variants Targeted:   ENSRNOT00000044635 ENSRNOT00000072121 ENSRNOT00000066739 ENSRNOT00000057461 ENSRNOT00000022864 ENSRNOT00000011922 ENSRNOT00000068312 ENSRNOT00000000891 ENSRNOT00000013378 ENSRNOT00000014354 ENSRNOT00000051235 ENSRNOT00000013515 ENSRNOT00000016784 ENSRNOT00000049793 ENSRNOT00000005405 ENSRNOT00000071383 ENSRNOT00000073303 ENSRNOT00000073321 ENSRNOT00000047908 ENSRNOT00000022983 ENSRNOT00000027682 ENSRNOT00000044183 ENSRNOT00000022382 ENSRNOT00000042547

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000030564
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000030564
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.800000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download