PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000045780, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000045780, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000045780, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0021399
Assay Design:   exonic
Chromosome Location:   7:14927269-14927401question
Amplicon Length:   103
Splice Variants Targeted:   ENSRNOT00000074120 ENSRNOT00000037756 ENSRNOT00000005006 ENSRNOT00000033551 ENSRNOT00000029694 ENSRNOT00000073387 ENSRNOT00000009280 ENSRNOT00000045636 ENSRNOT00000001527 ENSRNOT00000071130 ENSRNOT00000033969 ENSRNOT00000022358 ENSRNOT00000065282 ENSRNOT00000036189 ENSRNOT00000060284 ENSRNOT00000007428 ENSRNOT00000060210 ENSRNOT00000066095 ENSRNOT00000075589 ENSRNOT00000067074 ENSRNOT00000041034 ENSRNOT00000006335 ENSRNOT00000010427 ENSRNOT00000045356 ENSRNOT00000044558 ENSRNOT00000006235 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000045780
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000045780
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.300000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download