PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000050598, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000050598, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000050598, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0021395
Assay Design:   exonic
Chromosome Location:   7:19738571-19738717question
Amplicon Length:   117
Splice Variants Targeted:   ENSRNOT00000041244 ENSRNOT00000061907 ENSRNOT00000041532 ENSRNOT00000074923 ENSRNOT00000049266 ENSRNOT00000047547 ENSRNOT00000071844 ENSRNOT00000073174 ENSRNOT00000040119 ENSRNOT00000073222 ENSRNOT00000074792 ENSRNOT00000074699 ENSRNOT00000042693 ENSRNOT00000073754 ENSRNOT00000062058 ENSRNOT00000050916 ENSRNOT00000041082 ENSRNOT00000034630 ENSRNOT00000074428 ENSRNOT00000073410 ENSRNOT00000044505 ENSRNOT00000075323 ENSRNOT00000072621 ENSRNOT00000074130 ENSRNOT00000072076 ENSRNOT00000074598 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000050598
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050598
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.940000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download