PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000050222, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000050222, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000050222, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0021156
Assay Design:   exonic
Chromosome Location:   4:166974569-166974712question
Amplicon Length:   110
Splice Variants Targeted:   ENSRNOT00000017966 ENSRNOT00000058388 ENSRNOT00000075136 ENSRNOT00000058615 ENSRNOT00000063899 ENSRNOT00000045394 ENSRNOT00000002075 ENSRNOT00000074454 ENSRNOT00000074759 ENSRNOT00000011067 ENSRNOT00000048979 ENSRNOT00000058389 ENSRNOT00000071985 ENSRNOT00000040075 ENSRNOT00000048141 ENSRNOT00000042528 ENSRNOT00000008159 ENSRNOT00000055272 ENSRNOT00000065501 ENSRNOT00000071736 ENSRNOT00000072597 ENSRNOT00000074010 ENSRNOT00000075709 ENSRNOT00000074285 ENSRNOT00000058282 ENSRNOT00000047620 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000050222
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050222
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.990000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download