PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000048140, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000048140, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000048140, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0020361
Assay Design:   exonic
Chromosome Location:   20:4576287-4576454question
Amplicon Length:   138
Splice Variants Targeted:   ENSRNOT00000048858 ENSRNOT00000041074 ENSRNOT00000052311 ENSRNOT00000040634 ENSRNOT00000051480 ENSRNOT00000041500 ENSRNOT00000074123 ENSRNOT00000071931 ENSRNOT00000061415 ENSRNOT00000048535 ENSRNOT00000061395 ENSRNOT00000050159 ENSRNOT00000001035 ENSRNOT00000047439 ENSRNOT00000070903 ENSRNOT00000041502 ENSRNOT00000061114 ENSRNOT00000047527 ENSRNOT00000075244 ENSRNOT00000061297 ENSRNOT00000061027 ENSRNOT00000047779 ENSRNOT00000060980 ENSRNOT00000041151 ENSRNOT00000064734 ENSRNOT00000071840 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000048140
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048140
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.680000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download