PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000050261, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000050261, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000050261, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0020347
Assay Design:   exonic
Chromosome Location:   4:2033595-2033817question
Amplicon Length:   193
Splice Variants Targeted:   ENSRNOT00000074210 ENSRNOT00000074309 ENSRNOT00000075319 ENSRNOT00000071051 ENSRNOT00000074798 ENSRNOT00000071621 ENSRNOT00000048074 ENSRNOT00000072956 ENSRNOT00000075017 ENSRNOT00000074379 ENSRNOT00000050182 ENSRNOT00000075482 ENSRNOT00000072327 ENSRNOT00000074618 ENSRNOT00000071552 ENSRNOT00000072354

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000050261
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050261
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.990000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download