PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000050682, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000050682, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000050682, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0020256
Assay Design:   exonic
Chromosome Location:   5:81497952-81498379question
Amplicon Length:   90
Splice Variants Targeted:   ENSRNOT00000021609 ENSRNOT00000000146 ENSRNOT00000037901 ENSRNOT00000006969 ENSRNOT00000043056 ENSRNOT00000048383 ENSRNOT00000001349 ENSRNOT00000049316 ENSRNOT00000059374 ENSRNOT00000071395 ENSRNOT00000046857 ENSRNOT00000052175 ENSRNOT00000046760 ENSRNOT00000012293 ENSRNOT00000047961 ENSRNOT00000074349 ENSRNOT00000073954 ENSRNOT00000044186 ENSRNOT00000071926 ENSRNOT00000035486 ENSRNOT00000016196 ENSRNOT00000019824 ENSRNOT00000013262 ENSRNOT00000030378 ENSRNOT00000064571 ENSRNOT00000011551 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000050682
Gene Name:   Major urinary protein 15.5 kDa fatty acid-binding protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050682
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.570000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download