PrimePCR™ SYBR® Green Assay: Olr598, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0019515
Assay Design:   exonic
Chromosome Location:   3:82487948-82488084question
Amplicon Length:   107
Splice Variants Targeted:   ENSRNOT00000071529 ENSRNOT00000071912 ENSRNOT00000019246 ENSRNOT00000011549 ENSRNOT00000040603 ENSRNOT00000047008 ENSRNOT00000072235 ENSRNOT00000049264 ENSRNOT00000049471 ENSRNOT00000007665 ENSRNOT00000049950 ENSRNOT00000043371 ENSRNOT00000042996 ENSRNOT00000047110 ENSRNOT00000073455 ENSRNOT00000075375 ENSRNOT00000039963 ENSRNOT00000047702 ENSRNOT00000072050 ENSRNOT00000071348 ENSRNOT00000060094 ENSRNOT00000043307

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr598
Gene Name:   Olfactory receptor Olr598
Aliases:   Not Available
RefSeq:   NM_001000332
Ensembl:   ENSRNOG00000046501
Entrez:   295828
UniGene:   Rn.142880
Chromosome Mapping:   3q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.740000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download