PrimePCR™ SYBR® Green Assay: Hmg1l1, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0018669
Assay Design:   exonic
Chromosome Location:   19:70657166-70657306question
Amplicon Length:   111
Splice Variants Targeted:   ENSRNOT00000009426 ENSRNOT00000046984 ENSRNOT00000046053 ENSRNOT00000030194 ENSRNOT00000049646 ENSRNOT00000050478 ENSRNOT00000048281 ENSRNOT00000035711 ENSRNOT00000058640 ENSRNOT00000046997 ENSRNOT00000051642 ENSRNOT00000040376 ENSRNOT00000040837 ENSRNOT00000007793 ENSRNOT00000022114 ENSRNOT00000040905 ENSRNOT00000031497 ENSRNOT00000072831 ENSRNOT00000041628 ENSRNOT00000045504 ENSRNOT00000044787 ENSRNOT00000046626 ENSRNOT00000070854 ENSRNOT00000075275 ENSRNOT00000049438 ENSRNOT00000047765 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Hmg1l1
Gene Name:   Protein RGD1560584; RCG50935
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000034130
Entrez:   679571
UniGene:   Rn.4121,Rn.151172
Chromosome Mapping:   19q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.970000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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