PrimePCR™ SYBR® Green Assay: RGD1561722, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0018368
Assay Design:   exonic
Chromosome Location:   4:167392356-167392504question
Amplicon Length:   119
Splice Variants Targeted:   ENSRNOT00000058388 ENSRNOT00000008951 ENSRNOT00000005539 ENSRNOT00000064448 ENSRNOT00000050825 ENSRNOT00000011067 ENSRNOT00000058389 ENSRNOT00000064450 ENSRNOT00000057120 ENSRNOT00000071736 ENSRNOT00000031103 ENSRNOT00000074010 ENSRNOT00000074206 ENSRNOT00000073076 ENSRNOT00000075709 ENSRNOT00000074285 ENSRNOT00000047620 ENSRNOT00000074147 ENSRNOT00000074220 ENSRNOT00000074609 ENSRNOT00000073021

Gene Information

Description Not Available

Gene Symbol:   RGD1561722
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049185
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.890000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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