PrimePCR™ SYBR® Green Assay: Krtap9-1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Krtap9-1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Krtap9-1, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0018354
Assay Design:   exonic
Chromosome Location:   10:87571599-87571785question
Amplicon Length:   157
Splice Variants Targeted:   ENSRNOT00000018422 ENSRNOT00000045547 ENSRNOT00000008087 ENSRNOT00000017369 ENSRNOT00000063902 ENSRNOT00000064705 ENSRNOT00000001866 ENSRNOT00000050795 ENSRNOT00000050644 ENSRNOT00000012022 ENSRNOT00000046526 ENSRNOT00000017378 ENSRNOT00000042020 ENSRNOT00000014695 ENSRNOT00000054974 ENSRNOT00000064483 ENSRNOT00000015498 ENSRNOT00000017415 ENSRNOT00000044342

Gene Information

Description Not Available

Gene Symbol:   Krtap9-1
Gene Name:   Keratin associated protein 9-1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000026803
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.890000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download