PrimePCR™ SYBR® Green Assay: Olr227, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0018337
Assay Design:   exonic
Chromosome Location:   1:155165776-155165884question
Amplicon Length:   79
Splice Variants Targeted:   ENSRNOT00000066508 ENSRNOT00000035009 ENSRNOT00000026303 ENSRNOT00000004646 ENSRNOT00000046414 ENSRNOT00000036140 ENSRNOT00000023236 ENSRNOT00000027346 ENSRNOT00000023302 ENSRNOT00000073363 ENSRNOT00000019894 ENSRNOT00000075628 ENSRNOT00000015993 ENSRNOT00000023478 ENSRNOT00000074366 ENSRNOT00000026202 ENSRNOT00000001975 ENSRNOT00000072530 ENSRNOT00000018440 ENSRNOT00000044020 ENSRNOT00000034969 ENSRNOT00000042914 ENSRNOT00000066433 ENSRNOT00000013255 ENSRNOT00000013297 ENSRNOT00000028907 ENSRNO

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr227
Gene Name:   Olfactory receptor Olr227
Aliases:   Not Available
RefSeq:   NM_001000203
Ensembl:   ENSRNOG00000049285
Entrez:   293370
UniGene:   Rn.141790
Chromosome Mapping:   1q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.860000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download