PrimePCR™ SYBR® Green Assay: RGD1561841, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0017929
Assay Design:   exonic
Chromosome Location:   2:157975113-157975241question
Amplicon Length:   99
Splice Variants Targeted:   ENSRNOT00000066950 ENSRNOT00000039731 ENSRNOT00000033733 ENSRNOT00000068762 ENSRNOT00000057049 ENSRNOT00000034773 ENSRNOT00000041674 ENSRNOT00000043610 ENSRNOT00000042248 ENSRNOT00000015408 ENSRNOT00000047507

Gene Information

Description Not Available

Gene Symbol:   RGD1561841
Gene Name:   Protein RGD1561841
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000029493
Entrez:   310388
UniGene:   Not Available
Chromosome Mapping:   2q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.280000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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