PrimePCR™ SYBR® Green Assay: Olr1362, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Olr1362, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Olr1362, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0017503
Assay Design:   exonic
Chromosome Location:   10:12195193-12195291question
Amplicon Length:   69
Splice Variants Targeted:   ENSRNOT00000023302 ENSRNOT00000024186 ENSRNOT00000025851 ENSRNOT00000074694 ENSRNOT00000024163 ENSRNOT00000043872 ENSRNOT00000004448 ENSRNOT00000051612 ENSRNOT00000021134 ENSRNOT00000004435 ENSRNOT00000073642 ENSRNOT00000073060 ENSRNOT00000034730 ENSRNOT00000025964 ENSRNOT00000074303 ENSRNOT00000041708 ENSRNOT00000060987 ENSRNOT00000049390 ENSRNOT00000071686 ENSRNOT00000073280 ENSRNOT00000060995 ENSRNOT00000060994 ENSRNOT00000073401 ENSRNOT00000041612 ENSRNOT00000041353

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr1362
Gene Name:   Olfactory receptor Olr1362
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000047553
Entrez:   363546
Chromosome Mapping:   10q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.100000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download