PrimePCR™ SYBR® Green Assay: Vom1r71, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Vom1r71, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Vom1r71, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0017110
Assay Design:   exonic
Chromosome Location:   4:152250003-152250123question
Amplicon Length:   91
Splice Variants Targeted:   ENSRNOT00000038203 ENSRNOT00000058702 ENSRNOT00000040442 ENSRNOT00000073632 ENSRNOT00000008357 ENSRNOT00000074184 ENSRNOT00000052126 ENSRNOT00000073366 ENSRNOT00000073173 ENSRNOT00000072219 ENSRNOT00000004876 ENSRNOT00000040059 ENSRNOT00000040980 ENSRNOT00000050154 ENSRNOT00000040163

Gene Information

Description Not Available

Gene Symbol:   Vom1r71
Gene Name:   Vomeronasal 1 receptor 71
Aliases:   Not Available
RefSeq:   NM_001009523
Ensembl:   ENSRNOG00000047851
Entrez:   494243
Chromosome Mapping:   4q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 36.130000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download