PrimePCR™ SYBR® Green Assay: Olr1437, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0016747
Assay Design:   exonic
Chromosome Location:   10:44221474-44221609question
Amplicon Length:   106
Splice Variants Targeted:   ENSRNOT00000022637 ENSRNOT00000015799 ENSRNOT00000038703 ENSRNOT00000023995 ENSRNOT00000075040 ENSRNOT00000050288 ENSRNOT00000071834 ENSRNOT00000072352 ENSRNOT00000071556 ENSRNOT00000043430 ENSRNOT00000042880 ENSRNOT00000047914 ENSRNOT00000073040 ENSRNOT00000047671 ENSRNOT00000044332 ENSRNOT00000052167 ENSRNOT00000071262 ENSRNOT00000075204 ENSRNOT00000072854 ENSRNOT00000072282 ENSRNOT00000048778 ENSRNOT00000074062 ENSRNOT00000073426 ENSRNOT00000013060

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr1437
Gene Name:   Olfactory receptor Olr1437
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048938
Entrez:   287325
UniGene:   Rn.142136
Chromosome Mapping:   10q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.720000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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