PrimePCR™ SYBR® Green Assay: RGD1562409, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RGD1562409, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGD1562409, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0016735
Assay Design:   exonic
Chromosome Location:   3:65643296-65643414question
Amplicon Length:   89
Splice Variants Targeted:   ENSRNOT00000048646 ENSRNOT00000037123 ENSRNOT00000006485 ENSRNOT00000044593 ENSRNOT00000043418 ENSRNOT00000049436 ENSRNOT00000006754 ENSRNOT00000044551 ENSRNOT00000014590 ENSRNOT00000073389 ENSRNOT00000064857 ENSRNOT00000019331 ENSRNOT00000073752 ENSRNOT00000070922 ENSRNOT00000046365 ENSRNOT00000047694 ENSRNOT00000047511 ENSRNOT00000047713 ENSRNOT00000051387 ENSRNOT00000051207 ENSRNOT00000041410 ENSRNOT00000043491

Gene Information

Description Not Available

Gene Symbol:   RGD1562409
Gene Name:   Protein RGD1562409
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000029423
Entrez:   499809
Chromosome Mapping:   3q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.770000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download