PrimePCR™ SYBR® Green Assay: Cyp2d5, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Cyp2d5, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Cyp2d5, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0016660
Assay Design:   exonic
Chromosome Location:   7:123601569-123602052question
Amplicon Length:   73
Splice Variants Targeted:   ENSRNOT00000027943 ENSRNOT00000064705 ENSRNOT00000033268 ENSRNOT00000056366 ENSRNOT00000009683 ENSRNOT00000011880 ENSRNOT00000046024 ENSRNOT00000050002 ENSRNOT00000044470 ENSRNOT00000012413 ENSRNOT00000038789 ENSRNOT00000019096 ENSRNOT00000065585 ENSRNOT00000027875

Gene Information

member of a P450 IID gene subfamily that may have been maintained through gene conversion [RGD, Feb 2006]

Gene Symbol:   Cyp2d5
Gene Name:   Cytochrome P450 2D10
Aliases:   Not Available
RefSeq:   NM_173304
Ensembl:   ENSRNOG00000029128
Entrez:   286963
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 37.280000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download