PrimePCR™ SYBR® Green Assay: RT1-T24-1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: RT1-T24-1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RT1-T24-1, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0015805
Assay Design:   exonic
Chromosome Location:   20:5380355-5380480question
Amplicon Length:   96
Splice Variants Targeted:   ENSRNOT00000048858 ENSRNOT00000048535 ENSRNOT00000048036 ENSRNOT00000047439 ENSRNOT00000041502 ENSRNOT00000048076 ENSRNOT00000044520 ENSRNOT00000049540 ENSRNOT00000075244 ENSRNOT00000061027 ENSRNOT00000047779 ENSRNOT00000041151 ENSRNOT00000064734 ENSRNOT00000071840 ENSRNOT00000050099 ENSRNOT00000049310 ENSRNOT00000052270 ENSRNOT00000038660 ENSRNOT00000041590

Gene Information

Description Not Available

Gene Symbol:   RT1-T24-1
Gene Name:   RT1 class I, locus T24, gene 1
Aliases:   Not Available
RefSeq:   NM_001008858
Ensembl:   ENSRNOG00000032596
Entrez:   361787
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.800000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download