PrimePCR™ SYBR® Green Assay: RGD1562929, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RGD1562929, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGD1562929, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0015629
Assay Design:   exonic
Chromosome Location:   9:117331858-117331971question
Amplicon Length:   84
Splice Variants Targeted:   ENSRNOT00000074210 ENSRNOT00000001265 ENSRNOT00000016316 ENSRNOT00000060007 ENSRNOT00000046621 ENSRNOT00000044452 ENSRNOT00000017235 ENSRNOT00000016538 ENSRNOT00000012902 ENSRNOT00000072956 ENSRNOT00000008071 ENSRNOT00000013733 ENSRNOT00000056321 ENSRNOT00000046791 ENSRNOT00000071210 ENSRNOT00000049138 ENSRNOT00000050803 ENSRNOT00000071995 ENSRNOT00000050291 ENSRNOT00000042619 ENSRNOT00000074618 ENSRNOT00000049571 ENSRNOT00000048356 ENSRNOT00000050384 ENSRNOT00000048123 ENSRNOT00000054860 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RGD1562929
Gene Name:   Protein RGD1562929
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050799
Entrez:   316745
Chromosome Mapping:   9q38

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.460000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download