PrimePCR™ SYBR® Green Assay: Rps10l1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0015350
Assay Design:   exonic
Chromosome Location:   6:84969091-84969208question
Amplicon Length:   88
Splice Variants Targeted:   ENSRNOT00000033907 ENSRNOT00000049221 ENSRNOT00000066037 ENSRNOT00000024178 ENSRNOT00000020366 ENSRNOT00000046453 ENSRNOT00000022748 ENSRNOT00000056195 ENSRNOT00000043078 ENSRNOT00000055410 ENSRNOT00000021699 ENSRNOT00000045006 ENSRNOT00000025221 ENSRNOT00000026792 ENSRNOT00000040278 ENSRNOT00000016204 ENSRNOT00000042029 ENSRNOT00000043250 ENSRNOT00000047113

Gene Information

Description Not Available

Gene Symbol:   Rps10l1
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000031299
Entrez:   100363439
UniGene:   Not Available
Chromosome Mapping:   6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.430000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download