PrimePCR™ SYBR® Green Assay: RT1-S2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0014991
Assay Design:   exonic
Chromosome Location:   20:5254337-5254448question
Amplicon Length:   82
Splice Variants Targeted:   ENSRNOT00000048858 ENSRNOT00000000990 ENSRNOT00000052311 ENSRNOT00000040634 ENSRNOT00000051480 ENSRNOT00000041500 ENSRNOT00000039155 ENSRNOT00000038725 ENSRNOT00000042866 ENSRNOT00000050246 ENSRNOT00000074123 ENSRNOT00000071931 ENSRNOT00000061415 ENSRNOT00000048535 ENSRNOT00000061395 ENSRNOT00000036147 ENSRNOT00000050159 ENSRNOT00000042882 ENSRNOT00000048036 ENSRNOT00000001035 ENSRNOT00000001036 ENSRNOT00000048076 ENSRNOT00000044520 ENSRNOT00000049540 ENSRNOT00000061114 ENSRNOT00000047527 ENSRNO

Gene Information

may play a role in antigen presentation [RGD, Feb 2006]

Gene Symbol:   RT1-S2
Gene Name:   RT1 class Ib, locus S2 precursor
Aliases:   Not Available
RefSeq:   NM_001008857
Ensembl:   ENSRNOG00000029001
Entrez:   24994
UniGene:   Rn.222446
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.980000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download