PrimePCR™ SYBR® Green Assay: RGD1560194, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RGD1560194, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGD1560194, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0014881
Assay Design:   exonic
Chromosome Location:   18:4358947-4359095question
Amplicon Length:   119
Splice Variants Targeted:   ENSRNOT00000029007 ENSRNOT00000036333 ENSRNOT00000048646 ENSRNOT00000037123 ENSRNOT00000009816 ENSRNOT00000049151 ENSRNOT00000049436 ENSRNOT00000006754 ENSRNOT00000044551 ENSRNOT00000014590 ENSRNOT00000073389 ENSRNOT00000016409 ENSRNOT00000013435 ENSRNOT00000034377 ENSRNOT00000064857 ENSRNOT00000019331 ENSRNOT00000013313 ENSRNOT00000002935 ENSRNOT00000073752 ENSRNOT00000052141 ENSRNOT00000050690 ENSRNOT00000041103 ENSRNOT00000031545 ENSRNOT00000046365 ENSRNOT00000047694 ENSRNOT00000039882 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RGD1560194
Gene Name:   Protein LOC100361311
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000031244
Entrez:   366784
Chromosome Mapping:   7q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.080000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download