PrimePCR™ SYBR® Green Assay: Gm10013, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gm10013, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gm10013, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0013691
Assay Design:   exonic
Chromosome Location:   1:222269936-222270079question
Amplicon Length:   114
Splice Variants Targeted:   ENSRNOT00000023874 ENSRNOT00000000554 ENSRNOT00000005786 ENSRNOT00000061014 ENSRNOT00000012310 ENSRNOT00000000787 ENSRNOT00000065827 ENSRNOT00000071116 ENSRNOT00000060149 ENSRNOT00000064910 ENSRNOT00000004834 ENSRNOT00000010683 ENSRNOT00000061786 ENSRNOT00000073812 ENSRNOT00000065023 ENSRNOT00000015571 ENSRNOT00000068102 ENSRNOT00000019687 ENSRNOT00000067341 ENSRNOT00000056206 ENSRNOT00000006034 ENSRNOT00000048141 ENSRNOT00000028847 ENSRNOT00000050914 ENSRNOT00000028645 ENSRNOT00000045314 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Gm10013
Gene Name:   Predicted gene 10013
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000024991
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.490000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download