PrimePCR™ SYBR® Green Assay: Olr1226, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0013197
Assay Design:   exonic
Chromosome Location:   4:1347627-1347765question
Amplicon Length:   109
Splice Variants Targeted:   ENSRNOT00000073027 ENSRNOT00000072049 ENSRNOT00000072823 ENSRNOT00000072554 ENSRNOT00000072782 ENSRNOT00000071676 ENSRNOT00000071307 ENSRNOT00000074250 ENSRNOT00000071217 ENSRNOT00000071412 ENSRNOT00000049709 ENSRNOT00000073800 ENSRNOT00000071709 ENSRNOT00000071964 ENSRNOT00000072062 ENSRNOT00000074713 ENSRNOT00000074963

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr1226
Gene Name:   Olfactory receptor Olr1226
Aliases:   Not Available
RefSeq:   NM_001000442
Ensembl:   ENSRNOG00000049320
Entrez:   300551
UniGene:   Rn.109772
Chromosome Mapping:   8q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.230000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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