PrimePCR™ SYBR® Green Assay: Hist1h2ail, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hist1h2ail, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hist1h2ail, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0013135
Assay Design:   exonic
Chromosome Location:   17:45470677-45470826question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000073035 ENSRNOT00000074667 ENSRNOT00000021703 ENSRNOT00000010637 ENSRNOT00000050223 ENSRNOT00000072019 ENSRNOT00000072119 ENSRNOT00000074024 ENSRNOT00000073188 ENSRNOT00000075332 ENSRNOT00000052073 ENSRNOT00000040906

Gene Information

Description Not Available

Gene Symbol:   Hist1h2ail
Gene Name:   Histone H3.1
Aliases:   Not Available
RefSeq:   NM_001013056
Ensembl:   ENSRNOG00000046144
Entrez:   291159
Chromosome Mapping:   17p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.320000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download