PrimePCR™ SYBR® Green Assay: 9330160F10Rik, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0012471
Assay Design:   exonic
Chromosome Location:   10:55385423-55385567question
Amplicon Length:   115
Splice Variants Targeted:   ENSRNOT00000021037 ENSRNOT00000035915 ENSRNOT00000060404 ENSRNOT00000024831 ENSRNOT00000027847 ENSRNOT00000009936 ENSRNOT00000010333 ENSRNOT00000041689 ENSRNOT00000050649 ENSRNOT00000058007 ENSRNOT00000037941 ENSRNOT00000008679 ENSRNOT00000016714 ENSRNOT00000057977 ENSRNOT00000033975 ENSRNOT00000017461 ENSRNOT00000019502 ENSRNOT00000006443 ENSRNOT00000022384 ENSRNOT00000008507 ENSRNOT00000057157

Gene Information

Description Not Available

Gene Symbol:   9330160F10Rik
Gene Name:   RIKEN cDNA 9330160F10 gene
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000037672
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.150000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download