PrimePCR™ SYBR® Green Assay: GPBP1L1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GPBP1L1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GPBP1L1, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0012457
Assay Design:   exonic
Chromosome Location:   5:139212450-139212641question
Amplicon Length:   162
Splice Variants Targeted:   ENSRNOT00000067603 ENSRNOT00000050789 ENSRNOT00000035034 ENSRNOT00000059524 ENSRNOT00000060309 ENSRNOT00000071128 ENSRNOT00000033832 ENSRNOT00000012263 ENSRNOT00000036717 ENSRNOT00000017371 ENSRNOT00000019076 ENSRNOT00000041470 ENSRNOT00000075582 ENSRNOT00000022008

Gene Information

Description Not Available

Gene Symbol:   GPBP1L1
Gene Name:   Vasculin-like protein 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000037595
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.610000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download