PrimePCR™ SYBR® Green Assay: Hist1h2ac, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0012425
Assay Design:   exonic
Chromosome Location:   17:58949634-58949726question
Amplicon Length:   63
Splice Variants Targeted:   ENSRNOT00000067150 ENSRNOT00000020794 ENSRNOT00000057227 ENSRNOT00000001517 ENSRNOT00000011906 ENSRNOT00000066181 ENSRNOT00000066367 ENSRNOT00000050526 ENSRNOT00000022965 ENSRNOT00000059328 ENSRNOT00000074677 ENSRNOT00000074717 ENSRNOT00000073997 ENSRNOT00000047696 ENSRNOT00000044268

Gene Information

Description Not Available

Gene Symbol:   Hist1h2ac
Gene Name:   Histone H2A type 2-A
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048624
Entrez:   306962
UniGene:   Not Available
Chromosome Mapping:   17q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.000000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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